Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Myosin 1b Participated in the Modulation of Hypoxia/Reoxygenation-Caused H9c2 Cell Apoptosis and Autophagy

doi: 10.1155/2022/5187304

Figure Lengend Snippet: Overexpression of myo1b reversed the H/R-resulted H9c2 cell apoptosis. (a) Following H/R stimulation, the myo1b mRNA level in H9c2 cells was tested via qRT-PCR. (b) Following vector or OE-myo1b transfection, the myo1b mRNA level in H9c2 cells was tested via qRT-PCR. After H/R stimulation and OE-myo1b transfection, H9c2 cell apoptosis was measured via TUNEL assay (c) and Annexin V-FITC/PI staining (d); the Bcl-2, Bax, and cleaved-caspase 3 protein levels in H9c2 cells were evaluated through western blotting (e). ∗∗ P < 0.01 vs. control group; ## P < 0.01 vs. vector group; P < 0.01 vs. H/R + vector group.

Article Snippet: After that, cells were fixed with 4% paraformaldehyde solution for 30 min, permeabilized with 0.3% Triton X-100 solution for 5 min, rinsed with phosphate buffer saline (PBS), and incubated with TUNEL working solution (One Step TUNEL Apoptosis Assay Kit, Beyotime Biotechnology, Shanghai) for 1 h at 37°C protected from light.

Techniques: Over Expression, Quantitative RT-PCR, Plasmid Preparation, Transfection, TUNEL Assay, Staining, Western Blot, Control

Journal: Analytical Cellular Pathology (Amsterdam)

Article Title: Myosin 1b Participated in the Modulation of Hypoxia/Reoxygenation-Caused H9c2 Cell Apoptosis and Autophagy

doi: 10.1155/2022/5187304

Figure Lengend Snippet: Silence of myo1b promoted H9c2 cell apoptosis. After si-NC or si-myo1b transfection, H9c2 cell apoptosis was measured via TUNEL assay (a) and Annexin V-FITC/PI staining (b); the Bcl-2, Bax, and cleaved-caspase 3 protein levels in H9c2 cells were evaluated through western blotting (c). ∗∗ P < 0.01 vs. si-NC group.

Article Snippet: After that, cells were fixed with 4% paraformaldehyde solution for 30 min, permeabilized with 0.3% Triton X-100 solution for 5 min, rinsed with phosphate buffer saline (PBS), and incubated with TUNEL working solution (One Step TUNEL Apoptosis Assay Kit, Beyotime Biotechnology, Shanghai) for 1 h at 37°C protected from light.

Techniques: Transfection, TUNEL Assay, Staining, Western Blot